Reporter

Part:BBa_K4673027:Design

Designed by: Chieh Yu Lee   Group: iGEM23_Taipei-KCISLK-V1   (2023-10-02)


lacZα is a common reporter gene within E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The lacZ_α is located between the lac operon sequences and the M13-pUC_fwd_primer in the pGEM-T EASY vector.


Source

The lacZα gene was one of the components of the pGEM-T EASY vector, which our team purchased from a company called Promega. https://worldwide.promega.com/products/pcr/pcr-cloning/pgem-t-easy-vector-systems/?catNum=A1360

References

Hamed, A. A., Khedr, M., & Abdelraof, M. (2020, December 2). Activation of lacz gene in escherichia coli dh5α via α-complementation mechanism for β-galactosidase production and its biochemical characterizations. Journal, genetic engineering & biotechnology. https://pubmed.ncbi.nlm.nih.gov/33263861/